Cysteine Promoted C-Terminal Hydrazinolysis of Native Peptides and Proteins

被引：62

作者：

Adams, Anna L. ^{[1
]}

Cowper, Ben ^{[1
]}

Morgan, Rachel E. ^{[1
]}

Premdjee, Bhavesh ^{[1
]}

Caddick, Stephen ^{[1
]}

Macmillan, Derek ^{[1
]}

机构：

[1] UCL, Dept Chem, London WC1H 0AJ, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 49期

基金：

英国工程与自然科学研究理事会; 英国惠康基金;

关键词：

acyl transfer; erythropoietin; hydrazides; native chemical ligation; peptides; ACTIVE ESTER CONDENSATION; CHEMICAL-SYNTHESIS; SITE; ERYTHROPOIETIN; HYDRAZIDES; EFFICIENT; LIGATION;

D O I：

10.1002/anie.201304997

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tagging the terminus: N→S acyl transfer in native peptides and proteins can be reliably intercepted with hydrazine. The method allows selective labeling and ligation, without recourse to the use of protein-splicing elements. NCL=native chemical ligation. © 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：13062 / 13066

页数：5

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