Quantitative phosphoproteomic analysis of the tumor necrosis factor pathway

被引：78

作者：

Cantin, GT ^{[1
]}

Venable, JD ^{[1
]}

Cociorva, D ^{[1
]}

Yates, JR ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 01期

关键词：

isoelectric focusing; IMAC; phosphoproteome; metabolic labeling; TNF; PKA; apoptosis;

D O I：

10.1021/pr050270m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein phosphorylation has become a focus of many proteomic studies due to the central role that it plays in biology. We combine peptide-based gel-free isoelectric focusing and immobilized metal affinity chromatography to enhance the detection of phosphorylation events within complex protein samples using LC-MS. This method is then used to carry out a quantitative phosphoproteomic analysis of the tumor necrosis factor (TNF) pathway using HeLa cells metabolically labeled with N-15-containing amino acids, where 145 phosphorylation sites were found to be up-regulated upon the activation of the TNF pathway.

引用

页码：127 / 134

页数：8

共 43 条

[1] A multidimensional electrospray MS-based approach to phosphopeptide mapping [J].

Annan, RS ;

Huddleston, MJ ;

Verma, R ;

Deshaies, RJ ;

Carr, SA .

ANALYTICAL CHEMISTRY, 2001, 73 (03) :393-404

[2] Large-scale characterization of HeLa cell nuclear phosphoproteins [J].

Beausoleil, SA ;

Jedrychowski, M ;

Schwartz, D ;

Elias, JE ;

Villén, J ;

Li, JX ;

Cohn, MA ;

Cantley, LC ;

Gygi, SP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (33) :12130-12135

[3] Automatic Quality Assessment of Peptide Tandem Mass Spectra [J].

Bern, Marshall ;

Goldberg, David ;

McDonald, W. Hayes ;

Yates, John R., III .

BIOINFORMATICS, 2004, 20 :49-54

[4] Caspase-mediated activation of PAK2 during apoptosis: proteolytic kinase activation as a general mechanism of apoptotic signal transduction? [J].

Bokoch, GM .

CELL DEATH AND DIFFERENTIATION, 1998, 5 (08) :637-645

[5] ADENOVIRUS-E1B 19-KDA AND BCL-2 PROTEINS INTERACT WITH A COMMON SET OF CELLULAR PROTEINS [J].

BOYD, JM ;

MALSTROM, S ;

SUBRAMANIAN, T ;

VENKATESH, LK ;

SCHAEPER, U ;

ELANGOVAN, B ;

DSAEIPPER, C ;

CHINNADURAI, G .

CELL, 1994, 79 (02) :341-351

[6] Ca2+-dependent protein kinase-A modulation of the plasma membrane Ca2+-ATPase in parotid acinar cells [J].

Bruce, JIE ;

Yule, DI ;

Shuttleworth, TJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (50) :48172-48181

[7] Heat shock protein 27 is a substrate of cGMP-dependent protein kinase in intact human platelets - Phosphorylation-induced actin polymerization caused by Hsp27 mutants [J].

Butt, E ;

Immler, D ;

Meyer, HE ;

Kotlyarov, A ;

Laass, K ;

Gaestel, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (10) :7108-7113

[8] Physiological phosphorylation of protein kinase A at Thr-197 is by a protein kinase A kinase [J].

Cauthron, RD ;

Carter, KB ;

Liauw, S ;

Steinberg, RA .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (03) :1416-1423

[9] TNF-R1 signaling: A beautiful pathway [J].

Chen, GQ ;

Goeddel, DV .

SCIENCE, 2002, 296 (5573) :1634-1635

[10] Fragmentation of phosphopeptides in an ion trap mass spectrometer [J].

DeGnore, JP ;

Qin, J .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1998, 9 (11) :1175-1188

← 1 2 3 4 5 →