Comparison of polymerase chain reaction and conventional culture for the detection of legionellae in cooling tower waters in Singapore

被引：17

作者：

Ng, DLK

Koh, BB

Tay, L

Heng, BH

机构：

[1] Food and Water Microbiol. Laboratory, Department of Pathology, Singapore General Hospital, Singapore

[2] Food and Water Microbiol. Laboratory, Department of Pathology, Singapore 169608, Outram Road

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 24卷 / 03期

关键词：

D O I：

10.1046/j.1472-765X.1997.00382.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A total of 80 cooling tower water samples were investigated for legionellae using both cultural and polymerase chain reaction (PCR) methods. PCR was performed with the Perkin Elmer EnviroAmp(TM) Legionella kit. Forty-seven samples (58.8%) were found positive by both methods; 29 samples (36.3%) were positive by PCR only, while four samples (5%) showed PCR inhibition despite the adoption of the more stringent sample preparation protocol especially designed to eliminate inhibitors.

引用

页码：214 / 216

页数：3

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