YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex

被引：44

作者：

Krause-Buchholz, Udo ^{[1
]}

Gey, Uta

Wuenschmann, Jana

Becker, Susanne

Roedel, Gerhard

机构：

[1] Tech Univ Dresden, Inst Genet, D-01062 Dresden, Germany

[2] Univ Konstanz, Abt Analyt Chem, Fachbereich Chem, D-78457 Constance, Germany

来源：

FEBS LETTERS | 2006年 / 580卷 / 11期

关键词：

pyruvate dehydrogenase complex; mitochondria; phosphoproteins; YIL042c; YOR090c; S; cerevisiae;

D O I：

10.1016/j.febslet.2006.04.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Saccharomyces cerevisiae the pyruvate dehydrogenase (PDH) complex is regulated by reversible phosphorylation of its Pda1p subunit. We here provide evidence that Pda1p is phosphorylated by the mitochondrial kinase Yil042cp. Deletion of YOR090c, encoding a putative mitochondrial phosphatase, results in a decreased PDH activity, indicating that Yor090cp acts as the corresponding PDH phosphatase. We demonstrate by means of blue native gel electrophoresis and tandem affinity purification that both enzymes are associated with the PDH complex. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2553 / 2560

页数：8

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