Virulence properties of Escherichia coli isolated from Australian dairy powder factory environments

被引：7

作者：

Duffy, L. L. ^{[1
]}

O'Callaghan, D. ^{[1
]}

McAuley, C. M. ^{[1
]}

Fegan, N. ^{[1
]}

Craven, H. M. ^{[1
]}

机构：

[1] Food Sci Australia, Tingalpa, DC 4173, Australia

来源：

INTERNATIONAL DAIRY JOURNAL | 2009年 / 19卷 / 03期

关键词：

MULTIPLEX PCR;

D O I：

10.1016/j.idairyj.2008.09.002

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A total of 298 environmental samples from 5 separate dairy powder factories (A-E) were analysed for the presence of Escherichia coli. E. coli was isolated from 80 of these samples to obtain 359 isolates. These isolates were tested for the presence of 12 virulence genes associated with entero pathogenic E. coli (EPEC), atypical EPEC, Shiga toxin (Stx)-producing E. coli (STEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and enteroaggregative E. coli (EAEC). A total of 4 isolates obtained from two different sampling points at Factory C were positive for the astA gene, indicative of EAEC. A further 2 isolates from one sample from Factory C were positive for the presence of eaeA and escV genes, indicative of atypical EPEC. No virulence genes were detected in the remaining 353 isolates. While E. coli was present within the environment of dairy powder processing factories the carriage of potential virulence genes was low. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.

引用

页码：178 / 179

页数：2

共 18 条

[1]

*AUSTR STAND, 1992, 176623 AUSTR STAND

[2]

*COD AL COMM, 2003, RISK PROF ENT E COL

[3] Distribution and expression of the astA gene (EAST1 toxin) in Escherichia coli and Salmonella [J].