Molecular cloning and sequence analysis of the chitinase gene from Bacillus thuringiensis serovar alesti

被引：29

作者：

Lin, Y ^{[1
]}

Xiong, G

机构：

[1] Huaqiao Univ, Dept Bioengn & Biotechnol, Fujian Quanzhou 362011, Peoples R China

[2] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Pesticide Biochem, Fuzhou 350002, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 08期

关键词：

Bacillus thuringiensis; chitinase; molecular cloning; sequence analysis;

D O I：

10.1023/B:BILE.0000023021.50213.ed

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Endogenous chitinase plays a positive role in the pathogenicity of Bacillus thuringiensis to insect pests. The chitinase gene was cloned from B. thuringiensis serovar alesti strain HD-16, and the deduced 676 amino acid sequence showed a high degree of similarity with other Bacillus chitinases. Additionally, the deduced amino acid sequence showed that the protein contained an amino terminus signal peptide and consisted of a catalytic domain, a fibronectin type III domain and a chitin-binding domain. All three domains showed conserved sequences when compared to other bacterial chitinase or cellulase sequences.

引用

页码：635 / 639

页数：5

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