T cell responses to enterovirus antigens and to β-cell autoantigens in unaffected children positive for IDDM-associated autoantibodies

被引:24
作者
Juhela, S
Hyöty, H
Hinkkanen, A
Elliott, J
Roivainen, M
Kulmala, P
Rahko, J
Knip, M
Ilonen, J
机构
[1] Univ Turku, Dept Virol, FIN-20520 Turku, Finland
[2] Univ Turku, Turku Immunol Ctr, FIN-20520 Turku, Finland
[3] Tampere Univ, Sch Med, Dept Virol, FIN-33101 Tampere, Finland
[4] Tampere Univ Hosp, Tampere, Finland
[5] Abo Akad Univ, Dept Biochem & Pharm, SF-20500 Turku, Finland
[6] Univ Alberta, Dept Med Microbiol & Immunol, Edmonton, AB, Canada
[7] Natl Publ Hlth Inst, Dept Virol, Helsinki, Finland
[8] Oulu Univ, Dept Paediat, Oulu, Finland
[9] Tampere Univ, Sch Med, Dept Paediat, FIN-33101 Tampere, Finland
关键词
autoantigen; children; enteroviruses; IDDM; T cell proliferation;
D O I
10.1006/jaut.1999.0276
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Enterovirus infections have been implicated in the pathogenesis of IDDM in a number of studies. The aim of the present study was to evaluate whether the cellular immune response to enterovirus antigens is abnormal in children who test positive for IDDM-associated autoantibodies. Lymphocyte proliferation responses were analysed to enterovirus antigens and to a panel of beta-cell autoantigen preparations in 31 non-diabetic ICA and/or GAD65 antibody-positive children and in 19 ICA/GAD65-negative control children. The responses to highly purified enteroviruses did not differ between autoantibody (AA)-positive and -negative subjects. However, proliferation responses to coxsackievirus-infected cell lysate, which also included non-structural proteins of the virus, were higher in AA-positive than in AA-negative subjects (P<0.05). This difference was most marked in children carrying the HLA-DQB1*02 allele (P=0.01). AA-positive subjects also had higher responses to one of the three GAD65 antigen preparations compared to AA-negative subjects (P<0.05). Proliferation responses to the adenovirus hexon protein did not differ between the groups. These results show that the increased responses to virus infected cell lysates are associated with early phases of beta-cell autoimmunity. (C) 1999 Academic Press.
引用
收藏
页码:269 / 278
页数:10
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