Do ionic charges in ESI MS provide useful information on macromolecular structure?

被引：134

作者：

Kaltashov, Igor A. ^{[1
]}

Abzalimov, Rinat R. ^{[1
]}

机构：

[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2008年 / 19卷 / 09期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/j.jasms.2008.05.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multiple charging is all intrinsic feature of electrospray ionization (EST) of macromolecules. While multiple factors influence the appearance of protein ion charge state distributions in EST mass spectra, physical dimensions of protein molecules in solution are the major determinants of the extent Of multiple charging. This article reviews the information that call be obtained by analyzing ionic charge state distributions in EST MS, as well as potential pitfalls and limitations of this powerful technique. We also discuss future areas of growth with particular emphasis oil applications ill Structural biology, biotechnology (protein-polymer conjugates), and nanomedicine.

引用

页码：1239 / 1246

页数：8

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