High-speed plasmid isolation using 96-well, size-exclusion filter plates

被引：4

作者：

Harris, D ^{[1
]}

Engelstein, M ^{[1
]}

Parry, R ^{[1
]}

Smith, J ^{[1
]}

Mabuchi, M ^{[1
]}

Leonard, J ^{[1
]}

机构：

[1] Millipore Corp, Danvers, MA 01923 USA

来源：

BIOTECHNIQUES | 2002年 / 32卷 / 03期

关键词：

D O I：

10.2144/02323pf02

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The accelerating pace of genomics analysis has, necessitated the abbreviation of DNA sample preparation protocols. We have developed a size-exclusion-based system for the rapid isolation of plasmid DNA in a 96-well microplate format. This high-speed protocol employs a modified alkaline lysis method for the preparation of the bacterial lysate, followed by three short vacuum filtration steps. Unlike traditional bind/wash/elute methods, there is no need to use chaotropic salts or ethanol. The samples are recovered from the top side of the MultiScreen(96)(R) PLASMID plates. Starting with bacterial cell pellets, the entire procedure for purifying the plasmid DNA can be performed in 30 min with a multichannel pipettor The high yields, reproducibility, and quality of the plasmids make this system a good choice for any cloning or DNA sequencing operation.

引用

页码：626 / +

页数：5

共 9 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2]

Engelstein M, 1998, Microb Comp Genomics, V3, P237

[3] Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].