Effects of alcohols on lipid bilayers with and without cholesterol: the dipalmitoylphosphatidylcholine system

被引：41

作者：

Rosser, MFN ^{[1
]}

Lu, HM ^{[1
]}

Dea, P ^{[1
]}

机构：

[1] Occidental Coll, Dept Chem, Los Angeles, CA 90041 USA

来源：

BIOPHYSICAL CHEMISTRY | 1999年 / 81卷 / 01期

基金：

美国国家科学基金会;

关键词：

phospholipid; cholesterol; subgel; interdigitation; DSC; fluorescence;

D O I：

10.1016/S0301-4622(99)00081-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential scanning calorimetry is a useful method to study the thermotropic phase transitions of a phospholipid bilayer. In the present study DSC is used to determine the effects of methanol and ethanol on DPPC and DPPC/2 mol% cholesterol bilayers. The biphasic effect of the main transition and the presence of an extra peak on the DSC cooling scans were observed above certain alcohol concentrations. In the presence of 2% cholesterol, the concentration at which the biphasic effect occurs is increased by both short-chain alcohols. 1,6-Diphenyl-1,3,5-hexatriene (DPH) is used as a fluorescent probe to directly determine the onset of interdigitation in these systems as reflected by a drop in the DPH fluorescence intensity. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：33 / 44

页数：12

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