Induction of renin in medial smooth muscle cells by balloon injury

被引：51

作者：

Iwai, N ^{[1
]}

Izumi, M ^{[1
]}

Inagami, T ^{[1
]}

Kinoshita, M ^{[1
]}

机构：

[1] VANDERBILT UNIV,SCH MED,DEPT BIOCHEM,NASHVILLE,TN 37212

来源：

HYPERTENSION | 1997年 / 29卷 / 04期

关键词：

renin; intima; balloon injury;

D O I：

10.1161/01.HYP.29.4.1044

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

We studied the expression of each component of the renin-angiotensin system (renin, angiotensin I-converting enzyme, angiotensinogen, and angiotensin II type I receptor) in balloon-injured rat carotid artery. We assessed the expression levels of the respective mRNAs by competitive polymerase chain reaction. Renin mRNA concentration was markedly increased 24 hours after balloon injury and remained higher than that in the control at 7 days after balloon injury. Angiotensin-converting enzyme mRNA concentration was decreased 24 hours after balloon injury and was increased at 14 days after balloon injury. No significant change in angiotensinogen mRNA concentration was observed throughout the study period. Angiotensin type I receptor mRNA concentration was increased beginning 3 days after balloon injury and remained higher than that in the control at 14 days after balloon injury. Immunohistochemical analysis showed that renin was transiently expressed in medial smooth muscle cells after balloon injury. Administration of quinapril markedly reduced neointimal formation and was accompanied by an attenuation of the increase in the concentrations of angiotensin type I receptor and angiotensin-converting enzyme mRNAs. The upregulation of renin mRNA in balloon-injured rat carotid artery preceded and may play a role in neointimal formation.

引用

页码：1044 / 1050

页数：7

共 29 条

[1]

BARAJAS L, 1986, LAB INVEST, V54, P361

[2] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[3]

CLOWES AW, 1983, LAB INVEST, V49, P327

[4]

CLOWES AW, 1983, LAB INVEST, V49, P208

[5]

CLOWES AW, 1986, LAB INVEST, V54, P295

[6] ANGIOTENSIN-II INDUCES SMOOTH-MUSCLE CELL-PROLIFERATION IN THE NORMAL AND INJURED RAT ARTERIAL-WALL [J].

DAEMEN, MJAP ;

LOMBARDI, DM ;

BOSMAN, FT ;

SCHWARTZ, SM .

CIRCULATION RESEARCH, 1991, 68 (02) :450-456

[7] MECHANISM OF INHIBITION OF NEOINTIMAL FORMATION BY THE ANGIOTENSIN-CONVERTING ENZYME-INHIBITOR CILAZAPRIL - A STUDY IN BALLOON CATHETER-INJURED RAT CAROTID ARTERIES [J].

FINGERLE, J ;

MULLER, RMK ;

KUHN, H ;

PECH, M ;

BAUMGARTNER, HR .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1995, 15 (11) :1945-1950

[8] ROLE OF PLATELETS IN SMOOTH-MUSCLE CELL-PROLIFERATION AND MIGRATION AFTER VASCULAR INJURY IN RAT CAROTID-ARTERY [J].

FINGERLE, J ;

JOHNSON, R ;

CLOWES, AW ;

MAJESKY, MW ;

REIDY, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8412-8416

[9] FIBROBLAST GROWTH-FACTOR STIMULATES ANGIOTENSIN-CONVERTING ENZYME EXPRESSION IN VASCULAR SMOOTH-MUSCLE CELLS - POSSIBLE MEDIATOR OF THE RESPONSE TO VASCULAR INJURY [J].

FISHEL, RS ;

THOURANI, V ;

EISENBERG, SJ ;

SHAI, SY ;

CORSON, MA ;

NABEL, EG ;

BERNSTEIN, KE ;

BERK, BC .

JOURNAL OF CLINICAL INVESTIGATION, 1995, 95 (01) :377-387

[10] DISTRIBUTION OF RENIN MESSENGER-RNA AND ITS PROTEIN IN THE DEVELOPING KIDNEY [J].

GOMEZ, RA ;

LYNCH, KR ;

STURGILL, BC ;

ELWOOD, JP ;

CHEVALIER, RL ;

CAREY, RM ;

PEACH, MJ .

AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (05) :F850-F858

← 1 2 3 →