Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath)

被引：8

作者：

Csáki, R

Hanczár, T

Bodrossy, L

Murrell, JC

Kovács, KL

机构：

[1] Univ Szeged, Dept Biotechnol, H-6726 Szeged, Hungary

[2] Hungarian Acad Sci, Biol Res Ctr, Inst Biophys, H-6701 Szeged, Hungary

[3] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 205卷 / 02期

基金：

匈牙利科学研究基金会;

关键词：

membrane bound hydrogenase; hup gene cluster; NiFe] hydrogenase; methanotrophic bacterium; Methylococcus capsulatus (Bath);

D O I：

10.1016/S0378-1097(01)00469-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A Delta hupSL deletion mutant of Me. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：203 / 207

页数：5

共 27 条

[1] SEQUENCE-ANALYSIS OF SUBUNITS OF THE MEMBRANE-BOUND NITRATE REDUCTASE FROM A DENITRIFYING BACTERIUM - THE INTEGRAL MEMBRANE SUBUNIT PROVIDES A PROTOTYPE FOR THE DIHEME ELECTRON-CARRYING ARM OF A REDOX LOOP [J].