Complexity of Plasmodium falciparum Clinical Samples from Uganda during Short-Term Culture

被引：23

作者：

Nsobya, Samuel L. ^{[2
]}

Kiggundu, Moses ^{[2
]}

Joloba, Moses ^{[3
]}

Dorsey, Grant ^{[1
]}

Rosenthal, Philip J. ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA

[2] Makerere Univ, Dept Med, Kampala, Uganda

[3] Makerere Univ, Dept Microbiol, Kampala, Uganda

来源：

JOURNAL OF INFECTIOUS DISEASES | 2008年 / 198卷 / 10期

关键词：

D O I：

10.1086/592506

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We cultured Plasmodium falciparum parasites from 98 Ugandan children with malaria and determined the complexity of infection (COI) on the basis of msp-2 polymorphisms daily for 9 days. The mean COI decreased during culture from 1.73 to 1.56. New strains appeared after day 0 in 20 cultures. Strains disappeared after day 0 in 56% of 45 cultures that were initially mixed; persisting strains more commonly had wild-type dhfr (C59) and dhps (K540) sequences and mutant pfmdr1 (86Y) sequences. Thus, initial genotypes offer an imperfect representation of clinical COI. Loss of strains in culture may be due to diminished fitness of some drug-resistant strains.

引用

页码：1554 / 1557

页数：4

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