Adsorption dynamics of bovine serum albumin (BSA) onto binary interpenetrating polymer networks (IPNs) of poly(2-hydroxyethyl methacrylate) (PHEMA)

A binary hydrophilic interpenetrating polymer network (IPN) comprising of polyethylene glycol (PEG) and poly(2-hydroxyethyl methacrylate) (PHEMA) was prepared and the adsorption of bovine serum albumin (BSA) was carried out onto the surfaces of the IPNs. The adsorbed amounts of BSA were investigated as a function of the composition of the hydrogel, pH and ionic strength of the protein solution. The adsorption of protein was also studied kinetically and various parameters such as the rate constants for adsorption, diffusion and penetration rate constants were evaluated. For the assessment of the anti thrombogenic property of the IPNs surfaces, blood-clot formation tests were performed.