Molecular cloning and expression of the Na+/H+ exchanger gene in Oryza sativa

被引：176

作者：

Fukuda, A

Nakamura, A

Tanaka, Y

机构：

[1] Natl Inst Agrobiol Resources, Dept Plant Physiol, Ibaraki, Osaka 3058602, Japan

[2] Univ Tsukuba, Inst Biol Sci, Ibaraki, Osaka 305, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1446卷 / 1-2期

关键词：

OsNHX1; NHX1; Na+/H+ exchanger; salt stress; rice; Oryza sativa;

D O I：

10.1016/S0167-4781(99)00065-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Na+/H+ exchanger catalyzes the countertransport of Na+ and H+ across membranes. We isolated a rice cDNA clone the deduced amino acid sequence of which had homology with a putative Na+/H+ exchanger in Saccharomyces cerevisiae, NHX1. The sequence contains 2330 bp with an open reading frame of 1608 bp. The deduced amino acid sequence is similar to that of NHX1 and NHE isoforms in mammals, and shares high similarity with the sequences within predicted transmembrane segments and an amiloride-binding domain. The expression of the gene was increased by salt stress. These results suggest that the product of the novel gene, OsNHX1, functions as a Na+/H+ exchanger, and plays important roles in salt tolerance of rice. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：149 / 155

页数：7

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