Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb

被引：51

作者：

Morio, T ^{[1
]}

Hanissian, SH

Bacharier, LB

Teraoka, H

Nonoyama, S

Seki, M

Kondo, J

Nakano, H

Lee, SK

Geha, RS

Yata, J

机构：

[1] Tokyo Med & Dent Univ, Sch Med, Dept Pediat, Tokyo 1138519, Japan

[2] Childrens Hosp, Div Immunol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA

[4] Tokyo Med & Dent Univ, Med Res Inst, Dept Pathol Biochem, Tokyo 1010062, Japan

[5] Mitsubishi Chem Corp, Yokohama Res Ctr, Kanagawa 2278502, Japan

[6] Juntendo Univ, Sch Med, Dept Immunol, Tokyo 1138421, Japan

[7] Japan Sci & Technol Corp, Tokyo 1010062, Japan

[8] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

[9] Yonsei Univ, Bioprod Res Ctr, Seoul 120749, South Korea

来源：

IMMUNITY | 1999年 / 11卷 / 03期

关键词：

D O I：

10.1016/S1074-7613(00)80109-0

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

CD40 plays a critical role in survival, growth, differentiation, and class switching of B lymphocytes. Although Ku is required for immunoglobulin class switching, how CD40 signal transduction is coupled to Ku is still unknown. Here, we show that CD40 directly interacts with Ku through the membrane-proximal region of cytoplasmic CD40. Ku was confined to the cytoplasm in human primary B cells, and the engagement of CD40 on the B cells cultured in the presence of IL-4 resulted in the dissociation of Ku from CD40, translocation of Ku into the nucleus, and increase in the activity of DNA-dependent protein kinase. These findings indicate that Ku is involved in the CD40 signal transduction pathway and may play an important role in the CD40-mediated events.

引用

页码：339 / 348

页数：10

共 44 条

[1] Modulation of life and death by the TNF receptor superfamily [J].

Baker, SJ ;

Reddy, EP .

ONCOGENE, 1998, 17 (25) :3261-3270

[2] THE CD40 ANTIGEN AND ITS LIGAND [J].

BANCHEREAU, J ;

BAZAN, F ;

BLANCHARD, D ;

BRIERE, F ;

GALIZZI, JP ;

VANKOOTEN, C ;

LIU, YJ ;

ROUSSET, F ;

SAELAND, S .

ANNUAL REVIEW OF IMMUNOLOGY, 1994, 12 :881-922

[3] DNA-DEPENDENT ATPASE FROM HELA-CELLS IS RELATED TO HUMAN KU AUTOANTIGEN [J].

CAO, QP ;

PITT, S ;

LESZYK, J ;

BARIL, EF .

BIOCHEMISTRY, 1994, 33 (28) :8548-8557

[4] Ku80 is required for immunoglobulin isotype switching [J].

Casellas, R ;

Nussenzweig, A ;

Wuerffel, R ;

Pelanda, R ;

Reichlin, A ;

Suh, H ;

Qin, XF ;

Besmer, E ;

Kenter, A ;

Rajewsky, K ;

Nussenzweig, MC .

EMBO JOURNAL, 1998, 17 (08) :2404-2411

[5]

Chaudhuri A, 1997, J IMMUNOL, V159, P4244

[6] HOW B-CELLS AND T-CELLS TALK TO EACH OTHER [J].

CLARK, EA ;

LEDBETTER, JA .

NATURE, 1994, 367 (6462) :425-428

[7] DNA-DEPENDENT PROTEIN-KINASE ACTIVITY IS ABSENT IN XRS-6 CELLS - IMPLICATIONS FOR SITE-SPECIFIC RECOMBINATION AND DNA DOUBLE-STRAND BREAK REPAIR [J].

FINNIE, NJ ;

GOTTLIEB, TM ;

BLUNT, T ;

JEGGO, PA ;

JACKSON, SP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (01) :320-324

[8] Sequence-specific DNA binding by Ku autoantigen and its effects on transcription [J].

Giffin, W ;

Torrance, H ;

Rodda, DJ ;

Prefontaine, GG ;

Pope, L ;

Hache, RJG .

NATURE, 1996, 380 (6571) :265-268

[9] THE DNA-DEPENDENT PROTEIN-KINASE - REQUIREMENT FOR DNA ENDS AND ASSOCIATION WITH KU ANTIGEN [J].

GOTTLIEB, TM ;

JACKSON, SP .

CELL, 1993, 72 (01) :131-142

[10] Expression of DNA-dependent protein kinase holoenzyme upon induction of lymphocyte differentiation and V(D)J recombination [J].

Grawunder, U ;

Finnie, N ;

Jackson, SP ;

Riwar, B ;

Jessberger, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 241 (03) :931-940

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