Cytology by infrared micro-spectroscopy: Automatic distinction of cell types in urinary cytology

被引：34

作者：

Bird, Benjamin ^{[1
]}

Romeo, Melissa J. ^{[1
]}

Diem, Max ^{[1
]}

Bedrossian, Kristi ^{[2
]}

Laver, Nora ^{[2
]}

Naber, Stephen ^{[2
]}

机构：

[1] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA

[2] Tufts Univ, Sch Med, New England Med Ctr, Dept Pathol, Boston, MA 02111 USA

来源：

VIBRATIONAL SPECTROSCOPY | 2008年 / 48卷 / 01期

关键词：

cancer screening; infrared micro-spectroscopy; spectral cytology; principal component analysis (PCA); urine cytology;

D O I：

10.1016/j.vibspec.2008.03.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report microscopically collected infrared spectra of cells found in human urine in an effort to develop automatic methods for bladder cancer screening. Unsupervised multivariate analysis of the observed spectral patterns reveals distinct spectral classes, which correlate very well with visual cytology. Therefore, we believe that spectral analysis of individual cells can aid cytology in rendering reliable diagnoses based on objective measurements and discriminant algorithms. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：101 / 106

页数：6

共 22 条

[1] Conventional and liquid-based cervicovaginal cytology: A comparison study with clinical and histologic follow-up [J].

Baker, JJ .

DIAGNOSTIC CYTOPATHOLOGY, 2002, 27 (03) :185-188

[2] Microspectroscopy of single proliferating HeLa cells [J].

Boydston-White, S ;

Chernenko, T ;

Regina, A ;

Miljkovic, M ;

Matthäus, C ;

Diem, M .

VIBRATIONAL SPECTROSCOPY, 2005, 38 (1-2) :169-177

[3]

BRUNZEL NA, 1994, FUNDAMENTALS URINE B

[4] Infrared spectroscopic imaging: From planetary to cellular systems [J].

Colarusso, P ;

Kidder, LH ;

Levin, IW ;

Fraser, JC ;

Arens, JF ;

Lewis, EN .

APPLIED SPECTROSCOPY, 1998, 52 (03) :106A-120A

[5] Infrared spectroscopy of cells and tissues: Shining light onto a novel subject [J].

Diem, M ;

Boydston-White, S ;

Chiriboga, L .

APPLIED SPECTROSCOPY, 1999, 53 (04) :148A-161A

[6]

Diem M., 1993, Introduction to Modern Vibrational Spectroscopy

[7] Determining the utility and effectiveness of the NeoPath AutoPap 300 QC system used routinely [J].

Fetterman, B ;

Pawlick, G ;

Koo, H ;

Hartinger, J ;

Gilbert, C ;

Connell, S .

ACTA CYTOLOGICA, 1999, 43 (01) :13-22

[8]

Griffiths P. R., 2002, Handbook of Vibrational Spectroscopy

[9]

JACKSON M, 2000, ENCY ANAL CHEM, V1

[10]

Joliffe I.T., 1986, Principal Component Analysis

← 1 2 3 →