Genomic RNAi screening in Drosophila S2 cells:: what have we learned about host-pathogen interactions?

被引：45

作者：

Cherry, Sara ^{[1
]}

机构：

[1] Univ Penn, Dept Microbiol, Penn Genom Frontiers Inst, Philadelphia, PA 19104 USA

来源：

CURRENT OPINION IN MICROBIOLOGY | 2008年 / 11卷 / 03期

关键词：

D O I：

10.1016/j.mib.2008.05.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The detente between pathogen and host has been of keen interest to researchers in spite of being exceedingly difficult to probe. Recently, new RNA interference (RNAi) technologies, in particular in Drosophila tissue culture cells, have made it possible to interrogate the genetics of host organisms rapidly, with nearly complete genomic coverage and high fidelity. Therefore, it is not surprising that the applications of RNAi to the study of host-pathogen interactions were among the first to be published and have already revealed many new insights into the hosts' role in infection. This review will highlight the application of RNAi screening to pathogen-host interactions in Drosophila cells and will reveal some of the lessons learned from this approach.

引用

页码：262 / 270

页数：9

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