Tumour visualization in a murine model by time-delayed fluorescence of sulphonated aluminium phthalocyanine

Mice bearing the MS-2 fibrosarcoma were administered 0.25, 0.5 or 1 mg kg(-1) body weight (b.w.) of sulphonated aluminium phthalocyanine (AlS2Pc) (with average degree of sulphonation of 2.1), and time-gated fluorescence images were acquired up to 6 h after the injection. Different excitation wavelengths (610, 650 and 670 nm) were tested. Red light excitation and 3 ns delayed detection allow one to minimize natural fluorescence and scattered laser light, respectively. The best conditions for tumour detection are reached under either 650 or 670 nm excitation, 2-4 h after the administration of either 0.5 or 1 mg kg(-1) b.w. of AlS2Pc. In these situations, the average fluorescence contrast between tumour area and surrounding healthy tissue is >2, providing a clear identification of the pathological region. However, tumour localization is possible even after the injection of 0.25 mg kg(-1) b.w. of sensitizer. In conclusion, under low power excitation (<100 mu W cm(-2)), the technique allows real time detection of an intradermal tumour with good contrast.