Tissue engineering-based cartilage repair with allogenous chondrocytes and gelatin-chondroitin-hyaluronan tri-copolymer scaffold: A porcine model assessed at 18, 24, and 36 weeks

被引:98
作者
Chang, CH
Kuo, TF
Lin, CC
Chou, CH
Chen, KH
Lin, FH
Liu, HC
机构
[1] Natl Taiwan Univ Hosp, Dept Orthoped Surg, Taipei 100, Taiwan
[2] Natl Taiwan Univ, Inst Biomed Engn, Taipei 100, Taiwan
[3] Far Eastern Mem Hosp, Div Orthoped, Dept Surg, Taipei 220, Taiwan
[4] Natl Taiwan Univ, Vet Hosp, Taipei 10764, Taiwan
[5] Mid Formosa Univ Sci & Technol, Taichung, Taiwan
关键词
animal model; cartilage tissue engineering; gelatin; hyaluronan; chondroitin sulfate;
D O I
10.1016/j.biomaterials.2005.10.014
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We previously showed that cartilage tissue can be engineered in vitro with porcine chondrocytes and gelatin/chondoitin-6-sulfate/ hyaluronan tri-copolymer which mimic natural cartilage matrix for use as a scaffold. In this animal study, 15 miniature pigs were used in a randomized control study to compare tissue engineering with allogenous chondrocytes, autogenous osteochondral (OC) transplantation, and spontaneous repair for OC articular defects. In another study, 6 pigs were used as external controls in which full thickness (FT) and OC defects were either allowed to heal spontaneously or were filled with scaffold alone. After exclusion of cases with infection and secondary arthritis, the best results were obtained with autogenous OC transplantation, except that integration into host cartilage was poor. The results for the tissue engineering-treated group were satisfactory, the repair tissue being hyaline cartilage and/or fibrocartilage. Spontaneous healing and filling with scaffold alone did not result in good repair. With OC defects, the subchondral bone plate was not restored by cartilage tissue engineering. These results show that tri-copolymer can be used in in vivo cartilage tissue engineering for the treatment of FT articular defects. (C) 2005 Published by Elsevier Ltd.
引用
收藏
页码:1876 / 1888
页数:13
相关论文
共 32 条
[1]   Basic science of articular cartilage repair [J].
Athanasiou, KA ;
Shah, AR ;
Hernandez, RJ ;
LeBaron, RG .
CLINICS IN SPORTS MEDICINE, 2001, 20 (02) :223-+
[2]   Articular cartilage deformation under physiological cyclic loading-apparatus and measurement technique [J].
Barker, MK ;
Seedhom, BB .
JOURNAL OF BIOMECHANICS, 1997, 30 (04) :377-381
[3]  
Böstman OM, 2000, CLIN ORTHOP RELAT R, P216
[4]  
Breinan HA, 2001, CLIN ORTHOP RELAT R, pS219
[5]  
Buckwalter JA, 1999, CLIN ORTHOP RELAT R, pS224
[6]  
Carey J, 2000, J BIOMED MATER RES, V51, P711, DOI 10.1002/1097-4636(20000915)51:4<711::AID-JBM20>3.0.CO
[7]  
2-Z
[8]   Gelatin-chondroitin-hyaluronan tri-copolymer scaffold for cartilage tissue engineering [J].
Chang, CH ;
Liu, HC ;
Lin, CC ;
Chou, CH ;
Lin, FH .
BIOMATERIALS, 2003, 24 (26) :4853-4858
[9]   Chondrocyte transplantation using a collagen bilayer matrix for cartilage repair [J].
Frenkel, SR ;
Toolan, B ;
Menche, D ;
Pitman, MI ;
Pachence, JM .
JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 1997, 79B (05) :831-836
[10]  
Gilbert J E, 1998, Am J Knee Surg, V11, P42