Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture

被引：36

作者：

Wenger, J

Rigneault, H

Dintinger, J

Marguet, D

Lenne, PF

机构：

[1] Univ Aix Marseille 3, Inst Fresnel, CNRS, UMR 6133, F-13397 Marseille 20, France

[2] Univ Strasbourg 1, CNRS, UMR 7006, ISIS, F-67000 Strasbourg, France

[3] Ctr Immunol Marseille Luminy, CNRS, UMR 6102, INSERM,UMR 631, F-13009 Marseille, France

来源：

JOURNAL OF BIOLOGICAL PHYSICS | 2006年 / 32卷 / 01期

关键词：

fluorescence correlation spectroscopy; confocal microscopy; nano-apertures; lipid bilayer;

D O I：

10.1007/s10867-006-2909-x

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We use submicrometer apertures milled in an aluminium film to study the diffusion dynamics of beta-Bodipy-FL-C(5)-HPC (Bodipy-PC) fluorophores in a lipid dioleoylphosphatidylcholine (DOPC) multilayer. The observation volume is limited by the aperture diameter, well below the optical wavelength. This spatial resolution improvement comes together with an enhancement of the detected fluorescence per molecule as compared to an open sample, with a significant increase up to 3.5 times.

引用

页码：SN1 / SN4

页数：4

共 8 条

[1] Optical transmission properties of a single subwavelength aperture in a real metal [J].