Detection of potato mop-top virus capsid readthrough protein in virus particles

被引:30
作者
Cowan, GH [1 ]
Torrance, L [1 ]
Reavy, B [1 ]
机构
[1] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND
关键词
D O I
10.1099/0022-1317-78-7-1779
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Potato mop-top furovirus (PMTV) RNA 3 encodes the 20 kDa coat protein and a larger readthrough protein of 67 kDa. The readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. A 21 kDa C-terminal fragment of the readthrough protein was cloned, fused to glutathione S-transferase and expressed in E. coli. An antiserum prepared against purified fusion protein was used in ELISA to detect the readthrough protein in extracts of PMTV-infected leaves. Immunogold labelling studies showed that the readthrough protein was located near one extremity of some of the virus particles.
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收藏
页码:1779 / 1783
页数:5
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