Preproenkephalin, is a Th2 cytokine but is not required for Th2 differentiation in vitro

被引：22

作者：

Hook, S

Camberis, M

Prout, M

Konig, M

Zimmer, A

Van Heeke, G

Le Gros, G

机构：

[1] Wellington Sch Med, Malaghan Inst Med Res, Wellington, New Zealand

[2] NIMH, Genet Lab, Unit Mouse Genet, Bethesda, MD 20892 USA

[3] Novartis Horsham Res Ctr, Horsham, W Sussex, England

来源：

IMMUNOLOGY AND CELL BIOLOGY | 1999年 / 77卷 / 05期

基金：

英国惠康基金;

关键词：

IL-4; preproenkephalin; Th2 immune responses;

D O I：

10.1046/j.1440-1711.1999.00842.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Preproenkephalin (PPNK) mRNA expression has been detected in many cells of the immune system, including monocytes and lymphocytes. In the present paper. the expression of PPNK mRNA in purified CD4(+) Th1 and Th2 lymphocyte subpopulations is investigated and correlated with the presence of the opioid neuropeptides leu- and met-enkephalin. We found that PPNK mRNA and met-enkephalin were present at higher levels in the Th2 cultures compared with the Th1 cultures. Lymphocytes from PPNK-deficient mice were then used to look at the role of PPNK in Th2 lymphocyte differentiation. Lymphocytes from these mice could be driven into a Th2 phenotype, suggesting that cultures containing IL-4 do not require PPNK for Th2 differentiation.

引用

收藏

页码：385 / 390

页数：6

相关论文

共 19 条

[1] PROENKEPHALIN IS A NUCLEAR-PROTEIN RESPONSIVE TO GROWTH ARREST AND DIFFERENTIATION SIGNALS [J].

BOTTGER, A ;

SPRUCE, BA .

JOURNAL OF CELL BIOLOGY, 1995, 130 (06) :1251-1262

[2] OPIOID-PEPTIDES MODULATE PRODUCTION OF INTERFERON-GAMMA BY HUMAN MONONUCLEAR-CELLS [J].

BROWN, SL ;

VANEPPS, DE .

CELLULAR IMMUNOLOGY, 1986, 103 (01) :19-26

[3] 2 TYPES OF MOUSE HELPER T-CELL CLONE .3. FURTHER DIFFERENCES IN LYMPHOKINE SYNTHESIS BETWEEN TH1 AND TH2 CLONES REVEALED BY RNA HYBRIDIZATION, FUNCTIONALLY MONOSPECIFIC BIOASSAYS, AND MONOCLONAL-ANTIBODIES [J].

CHERWINSKI, HM ;

SCHUMACHER, JH ;

BROWN, KD ;

MOSMANN, TR .

JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 166 (05) :1229-1244

[4]

FONG TAT, 1990, J IMMUNOL, V144, P1744

[5] CONCENTRATION-DEPENDENT EFFECT OF MET-ENKEPHALIN ON HUMAN POLYMORPHONUCLEAR LEUKOCYTES [J].

FORIS, G ;

MEDGYESI, GA ;

NAGY, JT ;

VARGA, Z .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1987, 496 :151-157

[6] Real time quantitative PCR [J].

Heid, CA ;

Stevens, J ;

Livak, KJ ;

Williams, PM .

GENOME RESEARCH, 1996, 6 (10) :986-994

[7] T helper 2 cytokines induce preproenkephalin mRNA expression and proenkephalin A in human peripheral blood mononuclear cells [J].

Kamphuis, S ;

Kavelaars, A ;

Brooimans, R ;

Kuis, W ;

Zegers, BJM ;

Heijnen, CJ .

JOURNAL OF NEUROIMMUNOLOGY, 1997, 79 (01) :91-99

[8] Role of endogenous pro-enkephalin A-derived peptides in human T cell proliferation and monocyte IL-6 production [J].

Kamphuis, S ;

Eriksson, F ;

Kavelaars, A ;

Zijlstra, J ;

van de Pol, M ;

Kuis, W ;

Heijnen, CJ .

JOURNAL OF NEUROIMMUNOLOGY, 1998, 84 (01) :53-60

[9] Pain responses, anxiety and aggression in mice deficient in pre-proenkephalin [J].

Konig, M ;

Zimmer, AM ;

Steiner, H ;

Holmes, PV ;

Crawley, JN ;

Brownstein, MJ ;

Zimmer, A .

NATURE, 1996, 383 (6600) :535-538

[10] DIFFERENTIAL PROCESSING OF PROENKEPHALIN-A BY HUMAN PERIPHERAL-BLOOD MONOCYTES AND LYMPHOCYTES-T [J].

KUIS, W ;

VILLIGER, PM ;

LESER, HG ;

LOTZ, M .

JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (03) :817-824