Experience with a mouse intranasal test for the predictive identification of respiratory sensitization potential of proteins

被引:8
作者
Blaikie, L [1 ]
Basketter, DA [1 ]
机构
[1] Unilever Safety & Environm Assurance Ctr, Sharnbrook MK44 1LQ, Beds, England
关键词
respiratory; hypersensitivity; protein allergy; mouse intranasal test;
D O I
10.1016/S0278-6915(99)00068-X
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The predictive identification of respiratory allergenic potential is an important primary step in the safety evaluation of (novel) proteins, such as the enzymes used in a range of consumer laundry products. In the past this has been achieved by assessing the relative ability of proteins to give rise to the formation of anaphylactic antibody in the guinea pig. Recently, an alternative model has been proposed which assesses the formation of specific IgG1 antibody in a mouse intranasal test (MINT), the assumption being that specific IgG1 antibody is a surrogate for anaphylactic antibody in the mouse. This procedure has undergone successful initial intralaboratory and interlaboratory assessment. In the present work, the MINT has been evaluated in a more thorough intralaboratory study using eight enzymes plus ovalbumin. While the data generated with a reference enzyme protein, Alcalase, showed good reproducibility, results with the remaining eight proteins led to estimates of their relative antigenic or sensitization potential several of which were at variance from those derived from the guinea pig/human experience. In consequence, it is concluded that the MINT requires substantial further investigation before it can be adopted as a model for the assessment of the relative ability of proteins to behave as respiratory allergens. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:889 / 896
页数:8
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