Cellular mechanisms of β-amyloid production and secretion

The major constituent of senile plaques in Alzheimer's disease is a 42-aa peptide, referred to as beta-amyloid (A beta), A beta is generated from a family of differentially spliced, type-1 transmembrane domain (TM)-containing proteins, called APP, by endoproteolytic processing. The major, relatively ubiquitous pathway of APP metabolism in cell culture involves cleavage by alpha-secretase, which cleaves within the A beta sequence, thus precluding A beta formation and deposition, An alternate secretory pathway, enriched in neurons and brain, leads to cleavage of APP at the N terminus of the A beta peptide by beta-secretase, thus generating a cell-associated beta-C-terminal fragment (beta-CTF). A pathogenic mutation at codons 670/671 in APP (APP "Swedish") leads to enhanced cleavage at the beta-secretase scissile bond and increased A beta formation. An inhibitor of vacuolar ATPases, bafilomycin, selectively inhibits the action of beta-secretase in cell culture, suggesting a requirement for an acidic intracellular compartment for effective beta-secretase cleavage of APP, beta-CTF is cleaved in the TM domain by gamma-secretase(s), generating both A beta 1-40 (90%) and AP 1-42 (10%), Pathogenic mutations in APP at codon 717 (APP "London") lead to an increased proportion of A beta 1-42 being produced and secreted. Missense mutations in PS-1, localized to chromosome 14, are pathogenic in the majority of familial Alzheimer's pedigrees, These mutations also lead to increased production of A beta 1-42 over A beta 1-40, Knockout of PS-1 in transgenic animals leads to significant inhibition of production of both A beta 1-40 and A beta 1-42 in primary cultures, indicating that PS-1 expression is important for gamma-secretase cleavages, Peptide aldehyde inhibitors that block A beta production by inhibiting g-secretase cleavage of beta-CTF have been discovered.