Coagulase gene polymorphism of Staphylococcus aureus isolated from goat mastitis in Brazilian dairy herds

被引:17
作者
da Silva, ER
Boechat, JUD
da Silva, N
机构
[1] UFRPE, UAG, BR-55290000 Garanhuns, PE, Brazil
[2] Brazilian Res Goat Inst, Estrada Soral Groairas, Ceara, Brazil
[3] Univ Fed Minas Gerais, Sch Vet, Lab Mastitis Control, Belo Horizonte, MG, Brazil
关键词
coagulase gene polymorphism; goat; mastitis; restriction fragment length polymorphism; Staphylococcus aureus;
D O I
10.1111/j.1472-765X.2005.01799.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from mastitic goat's milk. Methods and Results: A typing procedure based on coagulase gene polymorphism was used to discriminate S. aureus isolated from goat mastitis. Thirty-six strains collected from goats belonging to herds from northern Ceara State and Serrana region of Rio de Janeiro State were analysed. Based on restriction fragment length polymorphism of 3' end coagulase gene, the goat strains were grouped into 11 types. In northern Ceara herds, the predominant type was found in 60% of the strains, while in the Serrana region herds the two most common accounting for 62.5% of the strains. Conclusions: The analysis of the coa gene proved to be useful for typing S. aureus from goat mastitis. Although the results showed that goats from the studied regions were infected by S. aureus strains harbouring more than one coagulase genotype, only one or two types predominated. Significance and Impact of the Study: The identification of the prevalent strains within a herd or region is a necessity. The important virulence factors could be identified in such strains and this information can then be used as a specific base to develop S. aureus mastitis control measures.
引用
收藏
页码:30 / 34
页数:5
相关论文
共 19 条
[1]  
AARESTRUP FM, 1995, CAN J VET RES, V59, P124
[2]   Development and use of PCR primers for the investigation of C1, C2 and C3 enterotoxin types of Staphylococcus aureus strains isolated from food-borne outbreaks [J].
Chen, TR ;
Hsiao, MH ;
Chiou, CS ;
Tsen, HY .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 71 (01) :63-70
[3]   Identification and in vitro antimicrobial susceptibility of Staphylococcus species isolated from goat mastitis in the Northeast of Brazil [J].
da Silva, ER ;
Siqueira, AP ;
Martins, JCD ;
Ferreira, WPB ;
da Silva, N .
SMALL RUMINANT RESEARCH, 2004, 55 (1-3) :45-49
[4]   STAPHYLOCOCCUS SPP AS MASTITIS-RELATED PATHOGENS IN GOAT MILK [J].
DEINHOFER, M ;
PERNTHANER, A .
VETERINARY MICROBIOLOGY, 1995, 43 (2-3) :161-166
[5]   Molecular population and virulence factor analysis of Staphylococcus aureus from bovine intramammary infection [J].
Fitzgerald, JR ;
Hartigan, PJ ;
Meaney, WJ ;
Smyth, CJ .
JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (06) :1028-1037
[6]   MOLECULAR TYPING OF STAPHYLOCOCCUS-AUREUS ON THE BASIS OF COAGULASE GENE POLYMORPHISMS [J].
GOH, SH ;
BYRNE, SK ;
ZHANG, JL ;
CHOW, AW .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1642-1645
[7]   NUMERICAL INDEX OF THE DISCRIMINATORY ABILITY OF TYPING SYSTEMS - AN APPLICATION OF SIMPSONS INDEX OF DIVERSITY [J].
HUNTER, PR ;
GASTON, MA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (11) :2465-2466
[8]   MASTITIS-RELATED PATHOGENS IN GOAT MILK [J].
KALOGRIDOUVASSILIADOU, D .
SMALL RUMINANT RESEARCH, 1991, 4 (02) :203-212
[9]   Molecular subtyping of Staphylococcus aureus isolates from cases of bovine mastitis in Brazil [J].
Lange, C ;
Cardoso, M ;
Senczek, D ;
Schwarz, S .
VETERINARY MICROBIOLOGY, 1999, 67 (02) :127-141
[10]  
LERONDELLE C, 1984, ANN RECH VET, V15, P105