Complement activation induced by purified Neisseria meningitidis lipopolysaccharide (LPS), outer membrane vesicles, whole bacteria, and an LPS-free mutant

被引:36
作者
Bjerre, A [1 ]
Brusletto, B
Mollnes, TE
Fritzsonn, E
Rosenqvist, E
Wedege, E
Namork, E
Kierulf, P
Brandtzæg, P
机构
[1] Ullevaal Univ Hosp, Dept Pediat, N-0407 Oslo, Norway
[2] Ullevaal Univ Hosp, Dept Clin Chem, N-0407 Oslo, Norway
[3] Natl Publ Hlth Inst, Dept Vaccinol, Oslo, Norway
[4] Natl Publ Hlth Inst, Dept Environm Med, Oslo, Norway
[5] Univ Tromso, Tromso, Norway
[6] Nordland Cent Hosp, Dept Immunol & Transfus Med, Bodo, Norway
关键词
D O I
10.1086/338269
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Complement activation is closely associated with plasma endotoxin levels in patients with meningococcal infections. This study assessed complement activation induced by purified Neisseria meningitidis lipopolysaccharide (Nm-LPS), native outer membrane vesicles (nOMVs), LPS-depleted outer membrane vesicles (dOMVs), wild-type meningococci, and an LPS-free mutant (lpxA(-)) from the same strain (44/76) in whole blood anticoagulated with the recombinant hirudin analogue. Complement activation products (C1rs-C1 inhibitor complexes, C4d, C3bBbP, and terminal SC5b-9 complex) were measured by double-antibody EIAs. Nm-LPS was a weak complement activator. Complement activation increased with preparations containing nOMVs, dOMVs, and wild-type bacteria at constant LPS concentrations. With the same protein concentration, complement activation induced by nOMVs, dOMVs, and the LPS-free mutant was equal. The massive complement activation observed in patients with fulminant meningococcal septicemia is, presumably, an indirect effect of the massive endotoxemia. Outer membrane proteins may be more potent complement activators than meningococcal LPSs.
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页码:220 / 228
页数:9
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