Bias in topoisomerase (TOPO)-cloning of multitemplate PCR products using locked nucleic acid (LNA)-substituted primers

被引：2

作者：

Liu, Yuan ^{[1
]}

Doering, Jessica ^{[1
]}

Hurek, Thomas ^{[1
]}

机构：

[1] Univ Bremen, Dept Microbe Plant Interact, CBIB, D-28359 Bremen, Germany

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 91卷 / 03期

关键词：

Cloning bias; LNA; PCR; TOPO; VACCINIA TOPOISOMERASE; LNA; DNA; OLIGONUCLEOTIDES; GENE; DISCRIMINATION; DESIGN; PROBES; PLANTS;

D O I：

10.1016/j.mimet.2012.10.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Locked nucleic acid (LNA) modifications help to improve nucleic acid recognition in molecular biology applications. We report that LNA-substituted primers in PCR reactions may cause considerable cloning bias when the widely used topoisomerase-based ligation is used for cloning of multitemplate PCR products. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：483 / 486

页数：4

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