Aspartic proteinase genes in the Brassicaceae Arabidopsis thaliana and Brassica napus

被引:37
作者
DHondt, K
Stack, S
Gutteridge, S
Vandekerckhove, J
Krebbers, E
Gal, S
机构
[1] SUNY BINGHAMTON,DEPT BIOL SCI,BINGHAMTON,NY 13902
[2] PLANT GENET SYST NV,B-9000 GHENT,BELGIUM
[3] STATE UNIV GHENT,LAB FYSIOL CHEM,B-9000 GHENT,BELGIUM
关键词
aspartic proteinase; gene isolation; intron/exon arrangement; protein processing; pulmonary surfactant-associated protein; saposin C;
D O I
10.1023/A:1005794917200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Active aspartic proteinase is isolated from Brassica napus seeds and the peptide sequence is used to generate primers for PCR. We present here cDNA and genomic clones for aspartic proteinases from the closely related Brassicaceae Arabidopsis thaliana and Brassica napus. The Arabidopsis cDNA represents a single gene, while Brassica has at least 4 genes. Like other plant aspartic proteases, the two Brassicaceae enzymes contain an extra protein domain of about 100 amino acids relative to the mammalian forms. The intron/exon arrangement in the Brassica genomic clone is significantly different from that in mammalian genes. As the proteinase is isolated from seeds, the same tissue where 2S albumins are processed, this implies expression of one of the aspartic proteinase genes there.
引用
收藏
页码:187 / 192
页数:6
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