Changes in cytokinin and auxin concentrations in seaweed concentrates when stored at an elevated temperature

被引:134
作者
Stirk, WA
Arthur, GD
Lourens, AF
Novák, O
Strnad, M
van Staden, J
机构
[1] Univ Natal Pietermaritzburg, Res Ctr Plant Growth & Dev, ZA-3209 Scottsville, South Africa
[2] Kelp Prod Pty Ltd, ZA-7995 Simonstown, South Africa
[3] Palacky Univ, Lab Growth Regulat, Olomouc 78371, Czech Republic
[4] ASCR, Inst Expt Bot, Olomouc 78371, Czech Republic
基金
新加坡国家研究基金会;
关键词
accelerated storage; auxins; cytokinins; Ecklonia maxima; Macrocystis pyrifera; seaweed concentrate;
D O I
10.1023/B:JAPH.0000019057.45363.f5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two seaweed concentrates were made from the kelps Ecklonia maxima and Macrocystis pyrifera using a cell burst method. Cytokinin- and auxin-like activities were measured using the soybean callus and mungbean bioassays, respectively. Cytokinin- like activity was detected in both seaweed concentrates, being equivalent to approximately 50 mug L-1 kinetin. Auxin-like activity was also detected in both concentrates with the E. maxima derived concentrate having higher biological activity, equivalent to 10(-5)-10(-4) M indole-butyric acid. Two replicates of each concentrate were stored at 54degreesC for 14 days to accelerate the effects of storage. Both fresh and stored samples of the two seaweed concentrates were analysed for their endogenous cytokinin and auxin content. The samples were purified using a combined DEAE-Sephadex octadecylsilica column and immunoaffinity chromatography based on wide-range cytokinin and IAA specific monoclonal antibodies. These extracts were analysed by HPLC linked to a Micromass single quadrupole mass spectrophotometer. Eighteen and nineteen different cytokinins were detected, respectively, in the two concentrates, with trans-zeatin-O-glucoside being the main cytokinin present. Accelerated storage of the concentrates caused an increase in the total cytokinin concentration with a large increase in the aromatic meta-topolin. Indole-3-acetic acid was the main auxin in both seaweed concentrates. Indole conjugates, including amino acid conjugates, were also quantified. The total auxin concentration decreased with accelerated storage for both concentrates.
引用
收藏
页码:31 / 39
页数:9
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