Strategies for generation of an siRNA expression library directed against the human genome

被引：84

作者：

Miyagishi, M

Taira, K ^{[1
]}

机构：

[1] Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Tokyo 1138656, Japan

[2] Natl Inst Adv Ind Sci & Technol, Gene Funct Res Ctr, Tsukuba, Ibaraki 3058562, Japan

来源：

OLIGONUCLEOTIDES | 2003年 / 13卷 / 05期

关键词：

D O I：

10.1089/154545703322617005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference (RNAi) is a phenomenon whereby expression of an individual gene is specifically silenced by the introduction of a double-stranded RNA (dsRNA) whose sequence is homologous to that of the gene in question. The generation of a small interfering RNA (siRNA) expression library directed against the entire human genome is a project that requires solutions to many difficult technical problems. We present here some strategies for solving some of these problems, including the development of genetically stable and highly active siRNA expression vectors, a procedure for selection of favorable target sites, and an efficient and inexpensive procedure for constructing an siRNA expression library.

引用

页码：325 / 333

页数：9

共 31 条

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