Nonylphenol enhances apoptosis induced by serum deprivation in PC12 cells

被引：68

作者：

Aoki, M

Kurasaki, M

Saito, T

Seki, S

Hosokawa, T

Takahashi, Y

Fujita, H

Iwakuma, T

机构：

[1] Hokkaido Univ, Grad Sch Environm Sci, Dept Environm Med & Informat, Kita Ku, Sapporo, Hokkaido 0600810, Japan

[2] Hokkaido Univ, Sch Med, Lab Environm Biol, Sapporo, Hokkaido 0608638, Japan

[3] Hokkaido Univ, Ctr Res & Dev Higher Educ, Res Div Higher Educ, Sapporo, Hokkaido 0600817, Japan

来源：

LIFE SCIENCES | 2004年 / 74卷 / 18期

基金：

日本学术振兴会;

关键词：

apoptosis; caspase; endocrine-disrupter; nonylphenol; PC12; cell; serum deprivation; Z-VAD;

D O I：

10.1016/j.lfs.2003.09.066

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

Although nonylphenol is well known as an endocrine disrupting chemical, there is little information concerning biological effect of nonylphenol. In this study, we investigated effect of nonylphenol on apoptosis induced by serum deprivation in PC 12 cells using TUNEL and DNA fragmentation assays. In addition, changes in contents of proapoptotic factors, Bad and Bax, and antiapoptotic factor, Bcl-2, and enzyme activity of caspase-3 were studied. Below 100 ng/ml of nonylphenol increased TUNEL signals, DNA fragmentation and content of proapoptotic factor, Bad as compared to those by serum deprivation without nonylphenol. Furthermore, addition of nonylphenol enhanced caspase-3 activity and Z-VAD, caspase-3 inhibitor, diminished such effect. These results indicated that below 100 ng/ml of nonylphenol enhanced apoptosis induced by serum deprivation via caspase-3 activation in PC12 cell. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：2301 / 2312

页数：12

共 38 条

[1]

Differential interaction of natural and synthetic estrogens with extracellular binding proteins in a yeast estrogen screen [J].