HPLC fractionation and structural dynamics of humic acids

被引：37

作者：

Woelki, G

Friedrich, S

Hanschmann, G

Salzer, R

机构：

[1] TECH UNIV DRESDEN, INST ANALYT CHEM, D-01062 DRESDEN, GERMANY

[2] CTR ENVIRON RES LEIPZIG HALLE LTD, D-04318 LEIPZIG, GERMANY

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1997年 / 357卷 / 05期

关键词：

D O I：

10.1007/s002160050211

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Different soil extracted humic acids as well as a commercial humic acid sodium salt were fractionated by HPLC. An almost complete recovery could be achieved for the dissolved material. All humic samples show a typical chromatogram of at least five fractions. The separation of humic substances is influenced by an altered hydrophobicity due to changes in the tertiary structure. Three fractions were further investigated by Diffuse Reflectance Infrared Fourier Transform (DRIFT) spectroscopy and by HPLC/HPLC separation. DRIFT spectroscopy provided data on the primary structure, whereas HPLC/HPLC results gave insight into the secondary and tertiary structure and their changes.

引用

页码：548 / 552

页数：5

共 19 条

[1]

ABBTBRAUN G, 1991, VOM WASSER, V77, P291

[2]

ABBTBRAUN G, 1993, REFRAKTARE ORGANISHC, P71

[3]

[Anonymous], 1990, BODENCHEMIE

[4]

FRENZ J, 1990, HPLC BIOL MACROMOLEC, P145

[5]

FRIMMEL FH, 1992, SCI TOTAL ENVIRON, V118, P197, DOI 10.1016/0048-9697(92)90087-9

[6]

GREMM T, 1991, VOM WASSER, V77, P231

[7]

HUBER S, 1990, VOM WASSER, V7, P331

[8] FOURIER-TRANSFORM INFRARED STUDIES OF COAL HUMIC ACIDS [J].

IBARRA, JV .

SCIENCE OF THE TOTAL ENVIRONMENT, 1989, 81-2 :121-128

[9] COMPARISON OF 2 METHODS OF PREPARATION OF HUMIC AND FULVIC-ACIDS, IHSS METHOD AND NAGOYA METHOD [J].

KUWATSUKA, S ;

WATANABE, A ;

ITOH, K ;

ARAI, S .

SOIL SCIENCE AND PLANT NUTRITION, 1992, 38 (01) :23-30

[10] EFFECTS OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SOLVENTS AND HYDROPHOBIC MATRICES ON THE SECONDARY AND QUATERNARY STRUCTURE OF A MODEL PROTEIN - REVERSED-PHASE AND SIZE-EXCLUSION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

LAU, SYM ;

TANEJA, AK ;

HODGES, RS .

JOURNAL OF CHROMATOGRAPHY, 1984, 317 (DEC) :129-140

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