Aluminium fluoride associates with the small guanine nucleotide binding proteins

被引：61

作者：

Ahmadian, MR

Mittal, R

Hall, A

Wittinghofer, A

机构：

[1] MAX PLANCK INST MOL PHYSIOL, D-44139 DORTMUND, GERMANY

[2] UNIV LONDON UNIV COLL, MRC, MOL CELL BIOL LAB, LONDON WC1E 6BT, ENGLAND

来源：

FEBS LETTERS | 1997年 / 408卷 / 03期

关键词：

aluminium fluoride; Ras; Cdc42; Rap; Ran;

D O I：

10.1016/S0014-5793(97)00422-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

AlF4- has long been known to associate with and activate the GDP-bound alpha subunits of heterotrimeric G-proteins. Recently the small guanine nucleotide binding protein Ras has also been shown to associate with AlF4- in the presence of stoichiometric amounts of its GTPase activating protein (GAP), Here we present the isolation of a stable Ras.GDP.AlF4-.GAP ternary complex by gel filtration, In addition, we generalise the association of AlF4- with the small GTP-binding proteins by demonstrating ternary complex formation for the Cdc42, Rap and Ran proteins in the presence of their respective GAP proteins. (C) 1997 Federation of European Biochemical Societies.

引用

页码：315 / 318

页数：4

共 29 条

[1] Structural differences in the minimal catalytic domains of the GTPase-activating proteins p120(GAP) and neurofibromin [J].