Suppression of the neoplastic phenotype by transfection of phospholipase C β 3 to neuroendocrine tumor cells

被引：8

作者：

Stålberg, P

Wang, S

Larsson, C

Weber, C

Öberg, K

Gobl, A

Skogseid, B ^{[1
]}

机构：

[1] Univ Uppsala Hosp, Dept Internal Med, S-75185 Uppsala, Sweden

[2] Karolinska Inst, Dept Mol Med, Clin Genet Unit, S-17176 Stockholm, Sweden

来源：

FEBS LETTERS | 1999年 / 450卷 / 03期

关键词：

phospholipase C beta 3; neuroendocrine tumor; transfection; xenograft; growth inhibition;

D O I：

10.1016/S0014-5793(99)00457-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of phospholipase C beta 3 (PLCB3) is low or absent in several neuroendocrine neoplasias. To investigate the role of PLCB3 in the neuroendocrine tumorigenesis, we transfected a PLCB3 construct to three neuroendocrine tumor cell lines with a low PLCB3 expression, The growth rate and tumorigenicity were assessed in vitro by [H-3]thymidine incorporation and cell counting, in vivo, by xenografting to nude mice. In vitro, PLCB3 expressing clones showed a significant growth inhibition. The tumor weight was reduced for one of the two xenografted PLCB3-transfected cell lines and in both, a reduced number of proliferating (Ki-67 positive) cells was observed. This study implies an essential role for PLCB3 in the neuroendocrine tumorigenesis, (C) 1999 Federation of European Biochemical Societies.

引用

页码：210 / 216

页数：7

共 28 条

[1] Isolation of 10 differentially expressed cDNAs in p53-induced apoptosis: Activation of the vertebrate homologue of the Drosophila seven in absentia gene [J].

Amson, RB ;

Nemani, M ;

Roperch, JP ;

Israeli, D ;

Bougueleret, L ;

LeGall, I ;

Medhioub, M ;

LinaresCruz, G ;

Lethrosne, F ;

Pasturaud, P ;

Piouffre, L ;

Prieur, S ;

Susini, L ;

Alvaro, V ;

Millasseau, P ;

Guidicelli, C ;

Bui, H ;

Massart, C ;

Cazes, L ;

Dufour, F ;

BruzzoniGiovanelli, H ;

Owadi, H ;

Hennion, C ;

Charpak, G ;

Dausset, J ;

Calvo, F ;

Oren, M ;

Cohen, D ;

Telerman, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :3953-3957

[2] Inhibition of human breast epithelial HBL100 cell proliferation by a dextran derivative (CMDB7) with the FGF2 autocrine loop [J].

BagheriYarmand, R ;

Liu, JF ;

Ledoux, D ;

Morere, JF ;

Crepin, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 239 (02) :424-428

[3] HYPERPARATHYROIDISM OF MULTIPLE ENDOCRINE NEOPLASIA TYPE-1 - CANDIDATE GENE AND PARATHYROID CALCIUM SENSING PROTEIN EXPRESSION [J].

CARLING, T ;

RASTAD, J ;

RIDEFELT, P ;

GOBL, A ;

HELLMAN, P ;

OBERG, K ;

RASK, L ;

LARSSON, C ;

JUHLIN, C ;

AKERSTROM, G ;

SKOGSEID, B .

SURGERY, 1995, 118 (06) :924-931

[4]

Chakrabarti R, 1998, GENE CHROMOSOME CANC, V22, P130, DOI 10.1002/(SICI)1098-2264(199806)22:2<130::AID-GCC7>3.0.CO

[5]

2-Y

[6] Positional cloning of the gene for multiple endocrine neoplasia-type 1 [J].

Chandrasekharappa, SC ;

Guru, SC ;

Manickam, P ;

Olufemi, SE ;

Collins, FS ;

EmmertBuck, MR ;

Debelenko, LV ;

Zhuang, ZP ;

Lubensky, IA ;

Liotta, LA ;

Crabtree, JS ;

Wang, YP ;

Roe, BA ;

Weisemann, J ;

Boguski, MS ;

Agarwal, SK ;

Kester, MB ;

Kim, YS ;

Heppner, C ;

Dong, QH ;

Spiegel, AM ;

Burns, AL ;

Marx, SJ .

SCIENCE, 1997, 276 (5311) :404-407

[7]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[8] DEMONSTRATION OF FUNCTIONALLY DIFFERENT INTERACTIONS BETWEEN PHOSPHOLIPASE C-GAMMA AND THE 2 TYPES OF PLATELET-DERIVED GROWTH-FACTOR RECEPTORS [J].

ERIKSSON, A ;

NANBERG, E ;

RONNSTRAND, L ;

ENGSTROM, U ;

HELLMAN, U ;

RUPP, E ;

CARPENTER, G ;

HELDIN, CH ;

CLAESSONWELSH, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (13) :7773-7781

[9] CONTINUOUS, CLONAL, INSULIN-SECRETING AND SOMATOSTATIN-SECRETING CELL-LINES ESTABLISHED FROM A TRANSPLANTABLE RAT ISLET CELL TUMOR [J].

GAZDAR, AF ;

CHICK, WL ;

OIE, HK ;

SIMS, HL ;

KING, DL ;

WEIR, GC ;

LAURIS, V .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (06) :3519-3523

[10] Somatic mutation of the MEN1 gene in parathyroid tumours [J].