Molecular cloning and characterization of a cDNA clone that encodes a Cdc2 homolog from Nicotiana tabacum

被引：29

作者：

Setiady, YY

Sekine, M

Hariguchi, N

Kouchi, H

Shinmyo, A

机构：

[1] NARA INST SCI & TECHNOL,GRAD SCH BIOL SCI,NARA 63001,JAPAN

[2] OSAKA UNIV,FAC ENGN,DEPT BIOTECHNOL,SUITA,OSAKA 565,JAPAN

[3] NATL INST AGROBIOL RESOURCES,TSUKUBA,IBARAKI 305,JAPAN

来源：

PLANT AND CELL PHYSIOLOGY | 1996年 / 37卷 / 03期

关键词：

Cdc2; homolog; cdc28(ts) yeast mutants; cdc2Nt1; Nicotiana tabacum (tobacco); proliferative-state-dependent expression;

D O I：

10.1093/oxfordjournals.pcp.a028955

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have isolated a cDNA clone (cdc2Nt1) that encodes a homolog of p34(cdc2/CDC28) kinase from tobacco (Nicotiana tabacum). The cdc2Nt1 protein showed extensive similarity to other homologs of Cdc2 from plants. Complementation studies showed that the cdc2Nt1 gene was able to overcome cell cycle arrest at both the G1/S and the G2/M transitions of cdc28(ts) mutants of budding yeast, demonstrating that the cdc2Nt1 protein was able to replace the Cdc28 kinase at both the G1/S and the G2/M transitions. Analysis of gene expression demonstrated that the cdc2Nt1 gene was transcribed constitutively throughout the cell cycle but that it was preferentially expressed in actively dividing tobacco BY-2 cells.

引用

页码：369 / 376

页数：8

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CHASAN R, 1995, PLANT CELL, V7, P1, DOI 10.1105/tpc.7.1.1

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