Bradykinin upregulates immediate-early gene mRNA in human keratinocytes

被引：13

作者：

Coutant, KD ^{[1
]}

Ryder, NS ^{[1
]}

机构：

[1] SANDOZ GMBH, RES INST, DEPT DERMATOL, A-1235 VIENNA, AUSTRIA

来源：

ARCHIVES OF DERMATOLOGICAL RESEARCH | 1996年 / 288卷 / 01期

关键词：

bradykinin; cell proliferation; epidermal growth factor; keratinocytes; primary response genes;

D O I：

10.1007/BF02505034

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

We investigated the effect of bradykinin on the expression of the proto-oncogenes c-fos, c-jun and c-myc and on cell proliferation in the human keratinocyte cell line, HaCaT. Analysis of mRNAs was done by Northern blotting with single-stranded DNA hybridization probes, Bradykinin caused a rapid and transient accumulation in c-fos and c-jun mRNAs. In contrast, c-myc mRNA increased more slowly, Moreover, we report that bradykinin was a weak stimulator of HaCaT cell proliferation whereas epidermal growth factor, which induced the same degree of mRNA elevation, was shown as a powerful mitogen, Thus, while in HaCaT cells bradykinin promotes the expression of the proto-oncogenes c-fos, c-jun and c-myc, other biochemical events appear to be necessary for cell division.

引用

页码：2 / 6

页数：5

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