UV-resonance Raman spectroscopic study of human plasma of healthy donors and patients with thrombotic microangiopathy

被引：30

作者：

Harz, M.

Claus, R. A.

Bockmeyer, C. L.

Baum, M.

Rosch, P.

Kentouche, K.

Deigner, H. -P

Popp, J.

机构：

[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[2] Univ Jena, Dept Anesthesia & Intens Care Med, D-07747 Jena, Germany

[3] Univ Jena, Dept Paediat, D-07743 Jena, Germany

来源：

BIOPOLYMERS | 2006年 / 82卷 / 04期

关键词：

cryoprecipitated human plasma; plasma components; UV-resonance Raman spectroscopy (UVRR);

D O I：

10.1002/bip.20489

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various diseases shift the composition of human plasma; hence, the relative quantification of plasma constituents offers the opportunity to use the dynamic and complex composition of plasma to gain information on novel diagnostic and prognostic factors. Since plasma contains, besides water, mostly proteins, UV-resonance Raman spectroscopy (UVRR) seems to be a suitable method for investigating plasma. With this method the signals of aromatic amino acids and proteins are selectively enhanced. In this study an UV-resonance Raman approach was used for the investigation of human plasma of healthy volunteers and patients with thrombotic microangiopathy. For comparison, selected plasma components were analyzed for a more detailed characterization of cryoprecipitates from human plasma. (c) 2006 Wiley Periodicals, Inc.

引用

页码：317 / 324

页数：8

共 20 条

[1] Ultralarge multimers of von Willebrand factor form spontaneous high-strength bonds with the platelet glycoprotein Ib-IX complex:: studies using optical tweezers [J].