A quantitative approach to catabolite repression in Escherichia coli

被引:107
作者
Bettenbrock, K
Fischer, S
Kremling, A
Jahreis, K
Sauter, T
Gilles, ED
机构
[1] Max Planck Inst Dynam Komplexer Tech Syst, Syst Biol Grp, D-39106 Magdeburg, Germany
[2] Univ Osnabruck, AG Genet, D-49069 Osnabruck, Germany
关键词
D O I
10.1074/jbc.M508090200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A dynamic mathematical model was developed to describe the uptake of various carbohydrates ( glucose, lactose, glycerol, sucrose, and galactose) in Escherichia coli. For validation a number of isogenic strains with defined mutations were used. By considering metabolic reactions as well as signal transduction processes influencing the relevant pathways, we were able to describe quantitatively the phenomenon of catabolite repression in E. coli. We verified model predictions by measuring time courses of several extra-and intracellular components such as glycolytic intermediates, EII-A(Crr) phosphorylation level, both LacZ and PtsG concentrations, and total cAMP concentrations under various growth conditions. The entire data base consists of 18 experiments performed with nine different strains. The model describes the expression of 17 key enzymes, 38 enzymatic reactions, and the dynamic behavior of more than 50 metabolites. The different phenomena affecting the phosphorylation level of EIIA(Crr), the key regulation molecule for inducer exclusion and catabolite repression in enteric bacteria, can now be explained quantitatively.
引用
收藏
页码:2578 / 2584
页数:7
相关论文
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