DNA single and double strand breaks induced by aliphatic and aromatic aldehydes in combination with copper(II)

被引：28

作者：

Becker, TW ^{[1
]}

Krieger, G ^{[1
]}

Witte, I ^{[1
]}

机构：

[1] CARL VON OSSIETZKY UNIV OLDENBURG,ICBM,FACHBEREICH 7,D-26111 OLDENBURG,GERMANY

来源：

FREE RADICAL RESEARCH | 1996年 / 24卷 / 05期

关键词：

aldehyde; copper; DNA strand breaks; genotoxicity; free radicals;

D O I：

10.3109/10715769609088030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aliphatic n-butyr- and n-valeraldehyde as well as the aromatic benz- and anisaldehyde induced DNA strand breaks in PM2 DNA in the presence of CuCl2. Neither aldehydes nor CuCl2 alone showed DNA breakage properties. The maximum of single strand breaks (SSBs) induced by the combination of CuCl2 and aldehydes was dependent on the CuCl2-concentration. The aliphatic aldehydes induced SSBs and double strand breaks (DSBs) at lower concentrations than aromatic aldehydes when optimal CuCl2 concentration were used. Catalase and neocuproine nearly completely inhibited strand break formation induced by aromatic aldehydes/CuCl2. The prevention of strand breaks induced by aliphatic aldehydes/CuCl2 was less effective. While the inhibition by neocuproine was only 25%, catalase was totally ineffective. In all aldehydes/CuCl2 mixtures the formation of Cu(I) was observed. The results point to different DNA damaging species produced during redox reactions of aromatic and aliphatic aldehydes in combination with CuCl2.

引用

页码：325 / 332

页数：8

共 31 条

[1]

AGARWAL K, 1989, CHEM-BIOL INTERACT, V65, P1

[2] ACTIVITIES OF FLAVONOIDS FOR THE CLEAVAGE OF DNA IN THE PRESENCE OF CU(II) - CORRELATION WITH GENERATION OF ACTIVE OXYGEN SPECIES [J].

AHMAD, MS ;

FAZAL, F ;

RAHMAN, A ;

HADI, SM ;

PARISH, JH .

CARCINOGENESIS, 1992, 13 (04) :605-608

[3] COPPER-DEPENDENT SITE-SPECIFIC MUTAGENESIS BY BENZOYL PEROXIDE IN THE SUPF GENE OF THE MUTATION REPORTER PLASMID PS189 [J].

AKMAN, SA ;

DOROSHOW, JH ;

KENSLER, TW .

CARCINOGENESIS, 1992, 13 (10) :1783-1787

[4] PARTITIONING OF ZINC AND COPPER WITHIN SUB-NUCLEAR NUCLEOPROTEIN PARTICLES [J].

BRYAN, SE ;

VIZARD, DL ;

BEARY, DA ;

LABICHE, RA ;

HARDY, KJ .

NUCLEIC ACIDS RESEARCH, 1981, 9 (21) :5811-5823

[5] INDUCTION AND MECHANISM OF DNA SINGLE-STRAND AND DOUBLE-STRAND BREAKS BY TETRACYCLINE/CU(II) IN THE ABSENCE OF LIGHT [J].

BUSCHFORT, C ;

WITTE, I .

CARCINOGENESIS, 1994, 15 (12) :2927-2930

[6] A SITE-SPECIFIC MECHANISM FOR FREE-RADICAL INDUCED BIOLOGICAL DAMAGE - THE ESSENTIAL ROLE OF REDOX-ACTIVE TRANSITION-METALS [J].

CHEVION, M .

FREE RADICAL BIOLOGY AND MEDICINE, 1988, 5 (01) :27-37

[7] DNA-SCISSION AND PROTEIN-SCISSION ACTIVITIES OF ASCORBATE IN THE PRESENCE OF COPPER-ION AND A COPPER-PEPTIDE COMPLEX [J].

CHIOU, SH .

JOURNAL OF BIOCHEMISTRY, 1983, 94 (04) :1259-1267

[8] DNA OF BACTERIOPHAGE PM2 - A CLOSED CIRCULAR DOUBLE-STRANDED MOLECULE [J].

ESPEJO, RT ;

CANELO, ES ;

SINSHEIMER, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 63 (04) :1164-+

[9] STRAND SCISSION IN DNA BY QUERCETIN AND CU(II) - IDENTIFICATION OF FREE-RADICAL INTERMEDIATES AND BIOLOGICAL CONSEQUENCES OF SCISSION [J].

FAZAL, F ;

RAHMAN, A ;

GREENSILL, J ;

AINLEY, K ;

HADI, SM ;

PARISH, JH .

CARCINOGENESIS, 1990, 11 (11) :2005-2008

[10] ALDEHYDES - OCCURRENCE, CARCINOGENIC POTENTIAL, MECHANISM OF ACTION AND RISK ASSESSMENT [J].

FERON, VJ ;

TIL, HP ;

DEVRIJER, F ;

WOUTERSEN, RA ;

CASSEE, FR ;

VANBLADEREN, PJ .

MUTATION RESEARCH, 1991, 259 (3-4) :363-385

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