Parallel picoliter RT-PCR assays using microfluidics

被引：136

作者：

Marcus, JS

Anderson, WF

Quake, SR

机构：

[1] CALTECH, Dept Appl Phys, Opt Biochem & Mol Biophys, Pasadena, CA 91125 USA

[2] Univ So Calif, Keck Sch Med, Gene Theapy Labs, Los Angeles, CA 90033 USA

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 03期

关键词：

D O I：

10.1021/ac0513865

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.

引用

页码：956 / 958

页数：3

共 15 条

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