A simple and accurate method for generating co-dominant markers: an application of conformation-sensitive gel electrophoresis to linkage analysis in the silkworm

被引：10

作者：

Yasukochi, Y ^{[1
]}

机构：

[1] Natl Inst Sericultural & Entomol Sci, Tsukuba, Ibaraki 3058634, Japan

来源：

MOLECULAR AND GENERAL GENETICS | 1999年 / 261卷 / 4-5期

基金：

日本科学技术振兴机构;

关键词：

conformation-sensitive gel electrophoresis heteroduplex formation; Bombyx mori; linkage analysis; sequence-tagged site;

D O I：

10.1007/s004380050023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive method for linkage analysis is described, which is based on conformation-sensitive gel electrophoresis (CSGE). Using urea-containing agarose gels or a commercially available polyacrylamide-derived matrix, 13 polymorphic markers were newly identified for known genes of the silkworm, Bombyx mori, which had been scored as monomorphic by PCR-RFLP analysis. This method for detecting polymorphisms is quite sensitive, and can be performed with inexpensive reagents and apparatus that is available in most molecular biology laboratories.

引用

页码：796 / 802

页数：7

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