Reduced expression of insulin-like growth factor binding protein-3 and its promoter hypermethylation in human hepatocellular carcinoma

被引：114

作者：

Hanafusa, T

Yumoto, Y

Nouso, K

Nakatsukasa, H

Onishi, T

Fujikawa, T

Taniyama, M

Nakamura, S

Uemura, M

Takuma, Y

Yumoto, E

Higashi, T

Tsuji, T

机构：

[1] Okayama Univ, Sch Med, Isotope Ctr, Okayama 7008558, Japan

[2] Okayama Univ, Sch Med, Dept Internal Med 1, Okayama 7008558, Japan

来源：

CANCER LETTERS | 2002年 / 176卷 / 02期

关键词：

cDNA microarray; hepatocellular carcinoma; insulin-like growth factor binding protein-3; hypermethylation; mannose 6-phosphate/insulin-like growth factor 2 receptor;

D O I：

10.1016/S0304-3835(01)00736-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Insulin-like growth factor binding protein-3 (IGFBP-3) is postulated to be a mediator of growth suppression signals. Reduced expression Of the IGFBP-3 was observed in nine out of 12 human hepatocellular carcinomas (HCC) (75%). Promoter hypermethylation of the IGFBP-3 was detected in four out of 12 HCCs (33%) although mutations were not identified. The expression of IGFBP-3 was restored by the demethylating agent 5-aza-2'-deoxycytidine in HCC cell line with promoter hypermethylation (HepG2). As IGFBP-3 functions like a tumor suppressor gene, it may be used as a therapeutic target for HCC. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：149 / 158

页数：10

共 37 条

[1] Alteration in pancreatic immunoreactivity of insulin-like growth factor (IGF)-binding protein (IGFBP)-6 and in intracellular degradation of IGFBP-3 in fibroblasts of IGF-II receptor/IGF-II-deficient mice [J].