Cloning and characterisation of an Arabidopsis thaliana cDNA clone encoding an organellar isoform of serine acetyltransferase

被引：28

作者：

Roberts, MA ^{[1
]}

Wray, JL ^{[1
]}

机构：

[1] UNIV ST ANDREWS,SCH BIOL & MED SCI,RES DIV ENVIRONM & EVOLUT BIOL,PLANT SCI LAB,ST ANDREWS KY16 9TH,FIFE,SCOTLAND

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 30卷 / 05期

关键词：

serine acetyltransferase; Arabidopsis thaliana; lambda YES; functional complementation; cysteine biosynthesis;

D O I：

10.1007/BF00020814

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned an Arabidopsis thaliana cDNA encoding serine acetyltransferase (EC 2.3.1.30) by functional complementation of the Escherichia coli cysE mutant JM15. The cDNA clone Sat-1 conferred serine acetyltransferase activity (with apparent K-m for the two substrates acetyl CoA and L-serine of 0.043 and 3.47 mmol/dm(3) respectively) on the cysE mutant. The 1515 bp full-length cDNA encodes a deduced protein of 391 amino acids which includes a putative chloroplastic targeting presequence. Northern analysis revealed a single message of 1.5 kb, while Southern hybridisation suggests a small multigene family of related sequences.

引用

页码：1041 / 1049

页数：9

共 53 条

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