Construction of mini-F plasmid vectors for plasmid shuffling in Escherichia coli

被引：21

作者：

Kato, J ^{[1
]}

Ikeda, H ^{[1
]}

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT MOLEC BIOL,TOKYO 108,JAPAN

来源：

GENE | 1996年 / 170卷 / 01期

关键词：

low-copy-number vector; mutagenesis; recombinant DNA tool;

D O I：

10.1016/0378-1119(95)00865-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed two Escherichia coli mini-F plasmid vectors, pJK286 and pJK289, which have unique EcoRI, BamHI and HindIII sites downstream from the lac promoter. The mini-F vectors are useful for plasmid shuffling, with which we can efficiently carry out localized mutagenesis.

引用

页码：141 / 142

页数：2

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