Challenges in the identification of species of canned fish

被引:127
作者
Mackie, IM [1 ]
Pryde, SE
Gonzales-Sotelo, C
Medina, I
Peréz-Martín, R
Quinteiro, J
Rey-Mendez, M
Rehbein, H
机构
[1] Rowett Res Inst, Aberdeen, Scotland
[2] Inst Invest Marinas Punta Betin, Vigo, Spain
[3] Univ Santiago de Compostela, Santiago De Compostela, Spain
[4] Bundesforschungsanstalt Fischerei, Inst Biochem & Technol, Hamburg, Germany
关键词
D O I
10.1016/S0924-2244(99)00013-8
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The identification of fish species becomes a problem when the usual identifying characteristics are removed on processing and only a portion of flesh is available. When the flesh is raw or cooked under normal conditions, the species is readily established by electrophoresis of the muscle proteins. The procedure cannot be used for heat-sterilised canned fish as the proteins are severely denatured. DNA is also degraded but techniques are now available for targeting and amplifying species-specific fragments. The amplified products can then be analysed by a range of techniques some of which are suitable for food control laboratories. (C) 1999 Elsevier Science Ltd. Ail rights reserved.
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页码:9 / 14
页数:6
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