The role of the QseC quorum-sensing sensor kinase in colonization and norepinephrine-enhanced motility of Salmonella enterica serovar Typhimurium

被引:148
作者
Bearson, Bradley L. [1 ]
Bearson, Shawn M. D. [2 ]
机构
[1] ARS, Swine Odor & Manure Management Res Unit, USDA, Natl Soil Tilth Lab, Ames, IA 50011 USA
[2] ARS, Preharvest Food Safety & Enter Dis Res Unit, USDA, Natl Anim Dis Ctr, Ames, IA 50011 USA
关键词
Salmonella; Quorum sensing; Colonization; Swine; Motility;
D O I
10.1016/j.micpath.2007.10.001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Transcriptional analysis of Salmonella enterica serovar Typhimurium (S. Typhimurium) in the presence of the mammalian hormone norepinephrine revealed Up-regulation of genes in the flagellar and chemotaxis regulon. Motility assays confirmed enhanced motility of wild-type S. Typhimurium in the presence of norepinephrine that Could be blocked by the alpha-adrenergic antagonist, phentolamine. Furthermore, a mutation in the qseC gene, encoding the sensor kinase of the two-component QseBC quorum-sensing system, also diminished motility of S. Typhimurium. To investigate the role of S. Typhimurium QseC in vivo, 13-week old pigs were intranasally inoculated with equal concentrations (1 x 10(9) CFU) of wild-type S. Typhimurium and a qseC mutant. Over a 1-week competitive index experiment, the qseC mutant displayed decreased colonization of the gastrointestinal tract compared to the wild-type parent strain. Thus, this study has identified a role for the QseBC quorum-sensing signal transduction system in motility and swine colonization of S. Typhimurium. Cross-talk between cell-cell communication systems in Salmonella (quorum sensing) and host hormones may explain opportunistic behaviors of the pathogen, such as immune evasion and stress-induced recrudescence of Salmonella, during fluctuations of host hormone levels. Published by Elsevier Ltd.
引用
收藏
页码:271 / 278
页数:8
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