F1C Fimbriae Play an Important Role in Biofilm Formation and Intestinal Colonization by the Escherichia coli Commensal Strain Nissle 1917

被引：100

作者：

Lasaro, Melissa A. ^{[2
]}

Salinger, Nina

Zhang, Jing ^{[3
]}

Wang, Yantao ^{[3
]}

Zhong, Zhengtao ^{[4
]}

Goulian, Mark ^{[2
]}

Zhu, Jun ^{[1
,4
]}

机构：

[1] Univ Penn, Sch Med, Dept Microbiol, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

[3] Nanjing Agr Univ, MOA Key Lab Microbiol Engn Agr Environm, Nanjing, Peoples R China

[4] Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Nanjing 210008, Peoples R China

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 01期

关键词：

URINARY-TRACT-INFECTION; TYPE-1; FIMBRIATION; PROTEUS-MIRABILIS; MOUSE MODEL; IDENTIFICATION; CONSTRUCTION; REMISSION; ADHERENCE; VIRULENCE; BACTERIA;

D O I：

10.1128/AEM.01144-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacterial biofilm formation is thought to enhance survival in natural environments and during interaction with hosts. A robust colonizer of the human gastrointestinal tract, Escherichia coli Nissle 1917, is widely employed in probiotic therapy. In this study, we performed a genetic screen to identify genes that are involved in Nissle biofilm formation. We found that F1C fimbriae are required for biofilm formation on an inert surface. In addition, these structures are also important for adherence to epithelial cells and persistence in infant mouse colonization. The data suggest a possible connection between Nissle biofilm formation and the survival of this commensal within the host. Further study of the requirements for robust biofilm formation may improve the therapeutic efficacy of Nissle 1917.

引用

页码：246 / 251

页数：6

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