Ultrastructural localization and regulation of protein gene product 9.5

被引：37

作者：

Lin, WM

Hsieh, ST

Huang, IT

Griffin, JW

Chen, WP

机构：

[1] NATL TAIWAN UNIV, COLL MED, DEPT ANAT, TAIPEI 10018, TAIWAN

[2] NATL TAIWAN UNIV, COLL MED, DEPT NEUROL, TAIPEI 10018, TAIWAN

[3] JOHNS HOPKINS UNIV, SCH MED, DEPT NEUROL, BALTIMORE, MD 21287 USA

[4] JOHNS HOPKINS UNIV, SCH MED, DEPT NEUROSCI, BALTIMORE, MD 21287 USA

来源：

NEUROREPORT | 1997年 / 8卷 / 14期

关键词：

axons; nerve degeneration; protein gene product 9.5; Schwann cells; ubiquitin hydrolase; Wallerian degeneration;

D O I：

10.1097/00001756-199709290-00002

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

PROTEIN gene product 9.5 (PGP), a ubiquitin hydrolase, is abundant in the nervous system. To investigate the ultrastructural localization of PGP and the regulation of its expression, we performed electron microscopic immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR) on normal and transected rat sciatic nerves. In normal nerves, strong PGP-immunoreactivity was localized in the myelinated and unmyelinated axons with virtually no staining in the Schwann cells. After nerve degeneration, denervated Schwann cells exhibited intense staining for PGP, corroborated with up-regulation of PGP transcripts by RT-PCR. The present data suggest that the pattern of expression of PGP is more complicated than was expected previously, and reflects the integrity of nerves and status of axon-Schwann cell interactions.

引用

页码：2999 / 3004

页数：6

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