Nitrogen mineralization in soil layers, soil particles and macro-organic matter under grassland

A study was conducted to determine mineralization rates in the: field and in different soil layers under three grassland managements (v-iz, a reseeded award, a permanent award with a conventional N management, and a long-term grass sward with 0 N (0-N) input). Potential mineralization rates of soil particles (sand, sill and clay) and macro-organic matter fractions of different sizes (i.e. 0.2-0.5, 0.5-2.0 and >2 mm) were also determined in the laboratory. In the reseeded plot-a, net mineralization was unchanged down to 40 cm depth. In the undisturbed conventional-N swards, mi neralization rates were substantially higher in the top layer (0-10 cm) than in the deeper layers, In plots which had received no fertilizer N, mineralization was consistently low in all the layers. There was more macro-organic matter (MOM) in the 0-N plots (equivalent to 23 g kg(-1) soil for 0-40 cm) than in the two-fertilized. plots (i.e. conventional-N and reseeded) which contained similar amounts (e.g. 15 g kg(-1) sail). C and N contents of separated soil particles did not differ amongst the treatments, but there were large differences with depth. Potential mineralization in the bulk soil was greatest in the 0-10 cm layers and gradually decreased with depth in all the treatments. Separated sand particles had negligible rates of potential mineralization and the clay component had the highest rates in the subsurface layers (10-40 cm). MOMs had high potential rate of mineralization in the surface layer and decreased with soil depth, hut there was no clear pattern in the differences between different size fractions.